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| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS12078 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-SENP3 |
| 克隆性 | 是 |
| 靶點(diǎn) | 詳見說(shuō)明書 |
| 適應(yīng)物種 | 詳見說(shuō)明書 |
| 形態(tài) | 詳見說(shuō)明書 |
| 宿主 | 詳見說(shuō)明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見說(shuō)明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human SENP3 |
產(chǎn)品訂購(gòu)信息：
英文名稱 Anti-SENP3
中文名稱 類泛素蛋白3抗體規(guī)格
別 名 Sentrin/SUMO specific protease 3; Sentrin specific protease 3; SMT3IP1; SSP3; SUMO1 specific protease 3; SUSP3; SENP3_HUMAN.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來(lái)源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Horse, Rabbit
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子
蛋白分子量 predicted molecular weight: 65kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human SENP3
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
類泛素蛋白3抗體規(guī)格 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The SENP3 protease releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. It deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability.
Function : Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1. Plays a role in the regulation of sumoylation status of ZNF148. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
Subunit : Binds to SUMO1 and SUMO3 (By similarity). Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with EP300, NPM1 and CDCA8. Component of the 5FMC complex, at least composed of PELP1, LAS1L, TEX10, WDR18 and SENP3; the complex interacts with methylated CHTOP and ZNF148. Interacts with NOL9.
Subcellular Location : Cytoplasm (By similarity). Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity). Redistributes between the nucleolus and the nucleoplasm in response to mild oxidative stress.
Similarity : Belongs to the peptidase C48 family.
Database links : UniProtKB/Swiss-Prot: Q9H4L4.2
CH ELISA Kit 大鼠膽固醇Multi-class antibodies規(guī)格： 48T
Anti-Phospho-TAK1 (Ser412) 磷酸化轉(zhuǎn)化生長(zhǎng)因子β活化激酶1Multi-class antibodies規(guī)格： 0.1ml
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5-HT(Mouse 5-Hydroxytryptamine) ELISA Kit 小鼠5羥色胺 96T
NTN3 英文名稱： 軸突生長(zhǎng)誘導(dǎo)因子3抗體 0.2ml
BMPER 英文名稱： BMP內(nèi)皮細(xì)胞調(diào)節(jié)蛋白抗體 0.2ml
Anti-Phospho-TAK1 (Ser412) 磷酸化轉(zhuǎn)化生長(zhǎng)因子β活化激酶1Multi-class antibodies規(guī)格： 0.1ml
Anti-EAAT1/Glast /FITC 熒光素標(biāo)記膠質(zhì)細(xì)胞谷氨酸運(yùn)載蛋白1 抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-MITF/FITC 熒光素標(biāo)記小眼相關(guān)轉(zhuǎn)錄因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh C4orf27 4號(hào)染色體開放閱讀框27抗體 規(guī)格 0.2ml
CD36 CD36(多肽) 0.5mg
HSP27 英文名稱： 熱休克蛋白27抗體 0.1ml
Rhesus antibody Rh SCF 干細(xì)胞生長(zhǎng)因子抗體 規(guī)格 0.1ml
Anti-MITF/FITC 熒光素標(biāo)記小眼相關(guān)轉(zhuǎn)錄因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml
ANG-1(Human Angiopoietin 1) ELISA Kit 人生成素1Multi-class antibodies規(guī)格： 48T
Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) 磷酸化p21激活激酶4、5、6抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh Hpt/Haptoglobin 結(jié)合珠蛋白/觸珠蛋白抗體 規(guī)格 0.1ml
ATGA/TGAB ELISA Kit 大鼠抗甲狀腺球蛋白抗體 96T
Phospho-PDGFRA(Tyr849)/PDGFRB(Tyr857) 英文名稱： 磷酸化血小板源性生長(zhǎng)因子受體α/β抗體 0.1ml
CACNB4 英文名稱： L型電壓依賴型鈣通道β4抗體 0.2ml
Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) 磷酸化p21激活激酶4、5、6抗體Multi-class antibodies規(guī)格： 0.1ml
FLT3 Others Mouse 小鼠 FLT-3 / CD135 / FLK-2 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
細(xì)胞凍存液CFM
人胃腺癌細(xì)胞;BGC-823
CCC-HEL-1細(xì)胞，人胚肝二倍體細(xì)胞 小鼠腹水瘤細(xì)胞,SAC-II B2細(xì)胞 EB病毒轉(zhuǎn)化的絨猴淋巴細(xì)胞;B95-8
MLTC-1(小鼠間質(zhì)細(xì)胞瘤細(xì)胞) 5×106cells/瓶×2
Promocell C-23110 Fibroblast Growth Medium KIT,成纖維細(xì)胞生長(zhǎng)培養(yǎng)基套裝 500ml
CL-0291MKN-28(人高轉(zhuǎn)移細(xì)胞)5×106cells/瓶×2
LTF Others Mouse 小鼠 Lactoansferrin / LTF 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
小鼠*導(dǎo)管上皮細(xì)胞完全培養(yǎng)基 100mL
PC14人細(xì)胞 PC14 human lung cancer cells DMEM+10% FBS
IL36RN Protein Mouse 重組小鼠 IL1F5 / IL1RP3 蛋白
P3/NSI/1-Ag4-1 [NS-1] (小鼠細(xì)胞) 5×106cells/瓶×2 MC3T3-E1 Subclone 14(小鼠原成骨細(xì)胞)
類泛素蛋白3抗體規(guī)格 CL-0288MA-891(小鼠癌高轉(zhuǎn)移細(xì)胞)5×106cells/瓶×2
CLEC4F Others Rat 大鼠 CLEC4F / CLECSF13 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
人前脂肪細(xì)胞-內(nèi)臟cDNAHPA-v cDNA
BCaP-37細(xì)胞，人癌細(xì)胞 猴腎細(xì)胞系,MA-104細(xì)胞 腸內(nèi)皮細(xì)胞Many types of cells包裝：5 × 105次方(1ml)
TE-13(人細(xì)胞) 5×106cells/瓶×2
HBdMEC Pellet 人膀胱微內(nèi)皮細(xì)胞團(tuán)塊 > 1 mio.cells 胰酶/EDTA消化液T/E
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 類泛素蛋白3抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及*基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定：
1）類泛素蛋白3抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
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