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| 產地 | 進口、國產 |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS11496 |
| 應用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-TGF Beta 1/2/3 |
| 克隆性 | |
| 靶點 | 詳見說明書 |
| 適應物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human TGF Beta 1 (301-350aa) |
抗體的生物素化標記實驗要點：
1. 轉化生長因子β1抗體說明書 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質之間的分子比按蛋白質表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；
5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性；
6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合；
7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產生競爭作用；
8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。
產品訂購信息：
英文名稱 Anti-TGF Beta 1/2/3
中文名稱 轉化生長因子β1抗體說明書
別 名 TGFβ1; TGFβ2; TGFβ3; CED; Diaphyseal dysplasia 1 progressive; DPD 1; DPD1; TGF beta 1; TGF beta; TGF beta1; TGF beta2; TGF beta3; TGF-beta 1; TGF-beta 2; TGF-beta 3; TGFB 1; TGFB; TGFB1; TGFB2; TGFB3; Transforming growth factor; Transforming growth factor beta 1; Transforming growth factor beta 2; Transforming growth factor beta 3; Transforming Growth Factor beta.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig
產品類型 一抗
研究領域 免疫學 信號轉導 生長因子和激素 轉錄調節(jié)因子
蛋白分子量 predicted molecular weight: 12.8/44kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human TGF Beta 1 (301-350aa)
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
轉化生長因子β1抗體說明書 產品應用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品介紹 The TGFBeta superfamily is composed of numerous growth and differentiation factors, including transforming growth factors Beta(TGFBeta)1,2 and 3; placental transforming growth factor (PTGFBeta);growth /differentiation factors (GDFs); mullerian inhibiting substance (MIS);bone morphogenic proteins (BMPs); glial cell line-derived neurotrophic factor (GDNF);inhibins or activins (Alpha,Beta-A, Beta-B and Beta-C),Lefty and Nodal. Members of the TGF superfamily are involved in embryonic development and adult tissue homeostasis.
Function : Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
Subunit : Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3. May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with CD109, DPT and ASPN.
Subcellular Location : Secreted, extracellular space, extracellular matrix.
Tissue Specificity : Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
Post-translational modifications : Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
DISEASE : Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
Similarity : Belongs to the TGF-beta family.
Database links : UniProtKB/Swiss-Prot: P01137.2
生長因子和激素（ Growth Factor and Hormones） TGF是一種多效生長因子，對上皮細胞增值有潛在，可抑制用于許多如:、、、、、等多種的研究。 TGFβ超級家族由為數眾多的生長的分化因子組成,包括轉移生長因子β1，2和3(TGF β1，TGF β2，TGF β3)；胎盤生長因子(PTGF-β)；生長/分化因子(GDFs)；繆氏抑制物(MIS)；骨形態(tài)形成蛋白(BMPs)；交織細胞元神經生長因子(GDNF);抑制素和活化素(α, β-A,和β-C)，Lefty和Nodal。 TGF超級家族成員參與胚胎發(fā)育和成體組織體內平衡。TGF-β1抗體與TGF-β2和TGF-β3沒有交叉反應。與豚鼠有大部分交叉.
抗體的鑒定：
1）轉化生長因子β1抗體說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質的識別能力。抗體的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質的交叉反應率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。
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Mouse Anti-rabbit IgG 小鼠抗兔IgG (親和層析純化)Multi-class antibodies規(guī)格： 1mg
谷氨酸半胱氨酸γ合成酶抗體 Anti-GCS 0.1ml
Donkey Anti-rabbit IgG/Alexa Fluor 555 Alexa Fluor 555標記的驢抗兔IgG 0.1ml
FKBP10 英文名稱： 肽基脯酰順反異構酶FKBP10抗體 0.2ml
Rhesus antibody Rh phospho-Smad3(Ser213) 磷酸化細胞信號轉導分子SMAD3抗體 規(guī)格 0.1ml
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Rhesus antibody Rh NUP160 核孔蛋白NUP160抗體 規(guī)格 0.2ml
Activiated Biotin 活化生物素 5mg
Tumstatin 英文名稱： 抑素抗體 0.2ml
Ceruloplasmin 英文名稱： 銅藍蛋白抗體 0.1ml
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MSTN Protein Mouse 重組小鼠 GDF-8 / Myostatin / MSTN 蛋白 (Fc 標簽)
BT474 人導管癌細胞
CPB2 Others Mouse 小鼠 Carboxypeptidase B2 / CPB2 人細胞裂解液 (陽性對照)
WI-38人胚肺細胞 WI-38 in human embryo lung cells MEM培養(yǎng)基(GIBCO)+10%FBS
tTA基因修飾的小鼠*癌細胞(B類);Pan02-CAG-tTA-4B3
人成纖維細胞(HPrF)(5×105) 人絨毛膜間充質基質細胞 Human
CM-H017人上皮細胞完全培養(yǎng)基100mL
ICOS Others Rat 大鼠 ICOS / AILIM / CD278 人細胞裂解液 (陽性對照)
主動脈內皮細胞Many types of cells包裝：5 × 105次方(1ml)
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MS751(人子宮頸表皮癌細胞) 5×106cells/瓶×2
BEL-7402(人細胞) 5×106cells/瓶×2 CM-R123大鼠角膜成纖維細胞完全培養(yǎng)基100mL 人細胞;Hela
轉化生長因子β1抗體說明書 MSTN Protein Mouse 重組小鼠 GDF-8 / Myostatin / MSTN 蛋白 (Fc 標簽)
BT474 人導管癌細胞
CPB2 Others Mouse 小鼠 Carboxypeptidase B2 / CPB2 人細胞裂解液 (陽性對照)
WI-38人胚肺細胞 WI-38 in human embryo lung cells MEM培養(yǎng)基(GIBCO)+10%FBS
tTA基因修飾的小鼠*癌細胞(B類);Pan02-CAG-tTA-4B3
人成纖維細胞(HPrF)(5×105) 人絨毛膜間充質基質細胞 Human
