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| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS11403 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-SAPAP1/DLGAP1 |
| 克隆性 | |
| 靶點(diǎn) | 詳見(jiàn)說(shuō)明書(shū) |
| 適應(yīng)物種 | 詳見(jiàn)說(shuō)明書(shū) |
| 形態(tài) | 詳見(jiàn)說(shuō)明書(shū) |
| 宿主 | 詳見(jiàn)說(shuō)明書(shū) |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書(shū) |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human SAPAP1/DLGAP1 |
產(chǎn)品訂購(gòu)信息：
英文名稱(chēng) Anti-SAPAP1/DLGAP1
中文名稱(chēng) PSD95結(jié)合蛋白1抗體品牌
別 名 PSD95 binding protein 1; DAP 1 ALPHA; DAP 1; DAP 1 BETA; DAP-1; DAP-1-ALPHA; DAP-1-BETA; DAP1; Discs large (Drosophila) homolog associated protein 1; Discs large homolog associated protein 1; Disks large associated protein 1; Disks large associated protein 1, alpha isoform, included; Disks large associated protein 1, beta isoform, included; Disks large-associated protein 1; DLGAP 1; Dlgap1; DLGP1_HUMAN; GKAP; GKAP/SAPAP; GKPA/SAPAP; Guanylate kinase associated protein; Guanylate kinase-associated protein; hGKAP; KIAA4162; MGC88156; mKIAA4162; PSD 95/SAP90 binding protein 1; PSD-95/SAP90-binding protein 1; SAP90/PSD 95 associated protein 1; SAP90/PSD-95-associated protein 1; SAPAP 1; SAPAP1.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來(lái)源 Rabbit
克隆類(lèi)型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep
產(chǎn)品類(lèi)型 一抗
研究領(lǐng)域 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 通道蛋白 細(xì)胞膜受體
蛋白分子量 predicted molecular weight: 109kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human SAPAP1/DLGAP1
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
PSD95結(jié)合蛋白1抗體品牌 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Members of the postsynaptic density-95 (PSD-95)/SAP90 family of membrane-associated guanylate kinase (MAGUK) proteins function as multimodular scaffolds that organize protein-signaling complexes at neuronal synapses. PSD-95/SAP90 binds guanylate kinase-associated protein (GKAP), also designated GK domain-binding protein, DAP-1-a, DAP-1-b, PSD-95 binding protein, PSD-95/SAP90 associated protein, or SAPAP, through the guanylate kinase domain. GKAP is expressed widely in neurons of the cortex and hippocampus and in the Purkinje and granule cells of the cerebellum. GKAP is localized specifically in the PSD of glutamatergic synapses, consistent with its direct interaction with PSD-95 family proteins.
Function : Part of the postsynaptic scaffold in neuronal cells.
Subunit : Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1 (By similarity).
Subcellular Location : Cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Found in postsynaptic density of neuronal cells.
Tissue Specificity : Expressed in brain.
Similarity : Belongs to the SAPAP family.
Database links : UniProtKB/Swiss-Prot: O14490.1
Anti-VEGF-D/FIGF/FITC 熒光素標(biāo)記兔抗人、大、小鼠內(nèi)皮生長(zhǎng)因子D型VEGFD抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Cdc2/P34CDC2/CDK1(Cell division control protein 2) 周期素依賴(lài)激酶1抗原Multi-class antibodies規(guī)格： 0.5mg
CD7抗體 Anti-CD7 0.1ml
TRIM21 英文名稱(chēng)： 核糖核蛋白抗原抗體 0.2ml
EID1 英文名稱(chēng)： 乙?；D(zhuǎn)移酶EID1抗體 0.2ml
Rhesus antibody Rh phospho-GPS1/CSN1(Ser474) 磷酸化G蛋白通路抑制蛋白1抗體 規(guī)格 0.1ml
Cdc2/P34CDC2/CDK1(Cell division control protein 2) 周期素依賴(lài)激酶1抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-TPO/FITC 熒光素標(biāo)記兔抗甲狀腺過(guò)氧化物酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml
PP-2B alpha 1 蛋酸酯酶-2B 催化亞單位(抗原)Multi-class antibodies規(guī)格： 0.5mg
抗甲狀腺過(guò)氧化物酶抗體 Anti-TPO 0.1ml
AP180 英文名稱(chēng)： 裝配銜接蛋白180抗體 0.2ml
EGR3 英文名稱(chēng)： 早期生長(zhǎng)反應(yīng)蛋白3抗體 0.1ml
Rhesus antibody Rh phospho-ITGB4(Tyr1530) 磷酸化整合素β4抗體 規(guī)格 0.1ml
PP-2B alpha 1 蛋酸酯酶-2B 催化亞單位(抗原)Multi-class antibodies規(guī)格： 0.5mg
pre-X protein[Hepatitis B virus]N-Terminus 乙毒pre-X蛋白抗原(N端)Multi-class antibodies規(guī)格： 0.5mg
Anti-Aurora A/B/C 有絲分裂激酶A/B/C抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh phospho-Akt(Thr308) 磷酸化蛋白激酶B抗體 規(guī)格 0.1ml
MBP 濃縮液 0.1ml 進(jìn)口分裝
UNC93B 英文名稱(chēng)： 內(nèi)質(zhì)網(wǎng)膜蛋白UNC93B抗體 0.2ml
Choline kinase alpha 英文名稱(chēng)： 乙醇胺激酶α抗體 0.1ml
Anti-Aurora A/B/C 有絲分裂激酶A/B/C抗體Multi-class antibodies規(guī)格： 0.2ml
IL10 Protein Human 重組人 IL10 / Ierleukin-10 蛋白
小鼠子宮內(nèi)膜上皮細(xì)胞完全培養(yǎng)基 100mL
PILRB Others Mouse 小鼠 PILRB1 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
H1650-M3人非小細(xì)胞細(xì)胞 H1650-M3 cells in human non small cell lung cancer 1640+10%FBS
CL-0031BEL-7402(人細(xì)胞)5×106cells/瓶×2
SK-N-BE(2) (人神經(jīng)母細(xì)胞瘤細(xì)胞) 5×106cells/瓶×2 U937(組織細(xì)胞)
CM-M055小鼠卵巢成纖維細(xì)胞完全培養(yǎng)基100mL
NAALADL1 Others Mouse 小鼠 NAALADL1 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
小鼠腎動(dòng)脈平滑肌細(xì)胞完全培養(yǎng)基 100mL
LLC小鼠Lewis細(xì)胞 LLC mice with Lewis lung cancer cells DMEM+10%FBS
IL12B Protein Mouse 重組小鼠 IL12B / IL-12B 蛋白
TE-10(人細(xì)胞) 5×106cells/瓶×2 HCC1428(人癌細(xì)胞)
PSD95結(jié)合蛋白1抗體品牌 IL10 Protein Human 重組人 IL10 / Ierleukin-10 蛋白
小鼠子宮內(nèi)膜上皮細(xì)胞完全培養(yǎng)基 100mL
PILRB Others Mouse 小鼠 PILRB1 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
H1650-M3人非小細(xì)胞細(xì)胞 H1650-M3 cells in human non small cell lung cancer 1640+10%FBS
CL-0031BEL-7402(人細(xì)胞)5×106cells/瓶×2
SK-N-BE(2) (人神經(jīng)母細(xì)胞瘤細(xì)胞) 5×106cells/瓶×2 U937(組織細(xì)胞)
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. PSD95結(jié)合蛋白1抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；
3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴(lài)氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及*基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定：
1）PSD95結(jié)合蛋白1抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
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