|
| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS11181 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-Raptor |
| 克隆性 | |
| 靶點(diǎn) | 詳見(jiàn)說(shuō)明書(shū) |
| 適應(yīng)物種 | 詳見(jiàn)說(shuō)明書(shū) |
| 形態(tài) | 詳見(jiàn)說(shuō)明書(shū) |
| 宿主 | 詳見(jiàn)說(shuō)明書(shū) |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書(shū) |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human Raptor |
mTOR相關(guān)調(diào)控蛋白抗體費(fèi)用 英文名稱 Anti-Raptor
中文名稱 mTOR相關(guān)調(diào)控蛋白抗體費(fèi)用
別 名 p150 target of rapamycin (TOR) scaffold protein containing WD repeats; P150 target of rapamycin (TOR)-scaffold protein; Regulatory Associated Protein of mTOR; KIAA1303; RPTOR_HUMAN; Regulatory-associated protein of mTOR; p150 target of rapamycin (TOR)-scaffold protein.
產(chǎn)品屬性：
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來(lái)源 Rabbit
克隆類(lèi)型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit
產(chǎn)品類(lèi)型 一抗
研究領(lǐng)域 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子
蛋白分子量 predicted molecular weight: 147kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Raptor
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
mTOR相關(guān)調(diào)控蛋白抗體費(fèi)用 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.
Function : Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
Subunit : Interacts with MTOR (By similarity). Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR and AKT1S1. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity.
Subcellular Location : Cytoplasm. Lysosome. Note=Targeting to lysosomes depends on amino acid availability.
Tissue Specificity : Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
Post-translational modifications : In response to nutrient limitation, phosphorylated by AMPK; phosphorylation promotes interaction with 14-3-3 proteins, leading to negative regulation of the mTORC1 complex. In response to growth factors, phosphorylated at Ser-719, Ser-721 and Ser-722 by RPS6KA1; phosphorylation positively stimulates mTORC1 activity.
Similarity : Belongs to the WD repeat RAPTOR family.
Contains 7 WD repeats.
Database links : UniProtKB/Swiss-Prot: Q8N122.1
乳類(lèi)雷帕霉素靶蛋白（mTOR）信號(hào)通路是涉及細(xì)胞增長(zhǎng)、、、和纖維化的重要信號(hào)通路，其相關(guān)分子Raptor也是目前的研究熱點(diǎn)。
實(shí)驗(yàn)流程：
全、新、優(yōu)、品、好四大特點(diǎn)：
mTOR相關(guān)調(diào)控蛋白抗體費(fèi)用 全：公司提供上萬(wàn)種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。
新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。
優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。
好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過(guò)程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒(méi)有任何的后顧之憂。
正在熱銷(xiāo)的相關(guān)產(chǎn)品：
NIT2 (nitrilase family, member 2) NIT2蛋白抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-Apollon/BIRC6 Apollon凋亡抑制蛋白抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh phospho-ATR/ACTR(Ser428) 磷酸化ATR抗體 規(guī)格 0.1ml
山羊抗小鼠 IgG(H+L)/AP 0.1ml 美國(guó)Jackson公司分裝
VSV-G tag 英文名稱： VSV-G tag標(biāo)簽抗體 0.1ml
DLST 英文名稱： DLST蛋白抗體 0.2ml
Anti-Apollon/BIRC6 Apollon凋亡抑制蛋白抗體Multi-class antibodies規(guī)格： 0.2ml
Anti-Phospho-PDGF Receptor beta (Tyr1009) /FITC 熒光素標(biāo)記磷酸化血小板源性生長(zhǎng)因子受體-B抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rabbit Anti-mouse IgG/FITC 熒光素標(biāo)記兔抗小鼠IgGMulti-class antibodies規(guī)格： 0.3ml
膠質(zhì)細(xì)胞谷氨酸運(yùn)載蛋白1 抗體 Anti-EAAT1/Glast 0.1ml
Rabbit Anti-human IgM/PE-Cy5.5 PE-Cy5.5標(biāo)記的兔抗人IgM 0.1ml
FGFR3 英文名稱： 成纖維細(xì)胞生長(zhǎng)因子受體3抗體 0.1ml
Rhesus antibody Rh Phospho-TIF1 beta(Ser824) 磷酸化轉(zhuǎn)錄中介因子Tif1β抗體 規(guī)格 0.1ml
Rabbit Anti-mouse IgG/FITC 熒光素標(biāo)記兔抗小鼠IgGMulti-class antibodies規(guī)格： 0.3ml
Synaptojanin 英文名稱： 磷酸肌醇磷酸酶蛋白INPP5G抗體 0.2ml
Estrogen receptor beta 英文名稱： 雌激素受體β抗體 0.1ml
組織蛋白酶L抗體 Anti-cath-L/CL 0.1ml
Anti-UCP-2/FITC 熒光素標(biāo)記線粒體脫偶連蛋白2抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh Phospho-Histone H3(Thr3) 磷酸化組蛋白H3抗體 規(guī)格 0.1ml
CCR-6/CD196 peptide 細(xì)胞表面趨化因子受體6抗原Multi-class antibodies規(guī)格： 0.5mg
RLFs, 大鼠
3T3? 成纖維細(xì)胞
CTSB Others Mouse 小鼠 Cathepsin-B / CTSB 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
CL-025*875(人細(xì)胞)5×106cells/瓶×2
人膀胱基質(zhì)成纖維細(xì)胞cDNAHBdSF cDNA
人胚腎細(xì)胞(亞系克隆);FIP293 人腎管狀上皮細(xì)胞完全培養(yǎng)基 100mL
CL-0146LTEP-a-2(人)5×106cells/瓶×2
IL27 Others Human 人 IL27 / Ierleukin-27 CHO細(xì)胞裂解液 (陽(yáng)性對(duì)照)
人尿道上皮細(xì)胞裂解物HUCL
長(zhǎng)白山豬胚胎皮膚成纖維樣細(xì)胞;201184 *癌細(xì)胞，CFPAC-1細(xì)胞 Ranca細(xì)胞，小鼠細(xì)胞
SV40T轉(zhuǎn)化的人胚腎細(xì)胞;293T
HA Others H1N1 甲型 H1N1 (A/Puerto Rico/8/1934) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
mTOR相關(guān)調(diào)控蛋白抗體費(fèi)用 RLFs, 大鼠
3T3? 成纖維細(xì)胞
CTSB Others Mouse 小鼠 Cathepsin-B / CTSB 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
CL-025*875(人細(xì)胞)5×106cells/瓶×2
人膀胱基質(zhì)成纖維細(xì)胞cDNAHBdSF cDNA
人胚腎細(xì)胞(亞系克隆);FIP293 人腎管狀上皮細(xì)胞完全培養(yǎng)基 100mL
技術(shù)外包服務(wù):
★mTOR相關(guān)調(diào)控蛋白抗體費(fèi)用 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等
★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。
|
|
|
|
|
