|
| 產(chǎn)地 | 進口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS11157 |
| 應用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-RhoA |
| 克隆性 | |
| 靶點 | 詳見說明書 |
| 適應物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human RhoA (101-150aa) |
技術外包服務:
★RhoA抗體費用 分子生物學：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學：基因合成、引物合成、基因測序、載體構建等
★蛋白工程：原核、哺乳動物蛋白表達系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細胞工程：細胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復、克隆形成)、細胞培養(yǎng)、細胞膜制備、穩(wěn)定細胞株構建、細胞RNAi技術等等。
實驗流程：
RhoA抗體費用 英文名稱 Anti-RhoA
中文名稱 RhoA抗體費用
別 名 RhoA protein; Aplysia ras related homolog 12; ARH 12; ARH12; ARHA; H 12; Oncogene RHO H12; Ras homolog gene family member A; RHO 12; Rho A; RHO H12; RHO12; RHOH 12; RHOH12; Small GTP binding protein Rho A; Small GTP binding protein RhoA; Transforming protein Rho A; Transforming protein RhoA.
產(chǎn)品屬性：
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Dog, Rabbit, Daniorerio
產(chǎn)品類型 一抗
研究領域 細胞生物 信號轉(zhuǎn)導 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 21kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human RhoA (101-150aa)
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
RhoA抗體費用 產(chǎn)品應用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Function : Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
Subunit : Interacts with ARHGEF28. Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts (in the constitutively activated, GTP-bound form) with DGKQ. Interacts with human respiratory syncytial virus (HRSV) protein F; this interaction facilitates virus-induced syncytium formation. Interacts with GNB2L1/RACK1; enhances RHOA activation. Interacts with PKP4; the interaction is detected at the midbody. Interacts (GTP-bound form preferentially) with PKN2; the interaction stimulates autophosphorylation and phosphorylation of PKN2.
Subcellular Location : Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex. Midbody. Note=Localized to cell-cell contacts in calcium-treated keratinocytes. Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner.
Post-translational modifications : Substrate for botulinum ADP-ribosyltransferase.
Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling.
Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
Similarity : Belongs to the small GTPase superfamily. Rho family.
RhoA蛋白主要分布在細胞質(zhì)和細胞膜,RhoA蛋白家族是控制細胞骨架蛋白組合、調(diào)節(jié)基因表達等細胞活動過程中的關鍵信號轉(zhuǎn)導成分。通常,RhoA通過激活其下游的Rho 連接激酶(Rho associated kinase,ROK)而起作用。在調(diào)節(jié)細胞骨架組建的過程中,RhoA與其作用對象均在胞質(zhì)內(nèi)。
全、新、優(yōu)、品、好四大特點：
RhoA抗體費用 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標準品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細胞等，基本上各種科研所需產(chǎn)品在我司都能找到。
新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。
優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。
好：我公司具有優(yōu)質(zhì)的技術團隊，產(chǎn)品一旦售出，實驗過程中遇到困難可提供在線技術咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。
正在熱銷的相關產(chǎn)品：
HO-1/HSP32 (Hemeo xygenase 1) 血紅素氧合酶-1/熱休克蛋白-32抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-AAT α-1抗胰蛋白酶抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh phospho-c-Jun(Thr91) 磷酸化原癌基因c-Jun抗體 規(guī)格 0.1ml
兔抗IgG血清 0.5ml 國產(chǎn)
WFS1 英文名稱： Wolfram綜合征蛋白1抗體 0.2ml
DRD5 英文名稱： 多巴胺受體D5抗體 0.1ml
Anti-AAT α-1抗胰蛋白酶抗體Multi-class antibodies規(guī)格： 0.1ml
Anti-PSAP/PAP/FITC 熒光素標記酸性磷酸酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rabbit Anti-rat IgG Whole serum 兔抗大鼠IgG抗血清Multi-class antibodies規(guī)格： 1ml
白介素8抗體 Anti-IL-8rat mouse 0.1ml
Rabbit anti-chicken IgM whole serum 兔抗雞IgM抗血清 1ml
FZD3 英文名稱： 卷曲蛋白3抗體 0.2ml
Rhesus antibody Rh phospho-PRL(Ser163) 磷酸化泌乳素抗體 規(guī)格 0.1ml
Rabbit Anti-rat IgG Whole serum 兔抗大鼠IgG抗血清Multi-class antibodies規(guī)格： 1ml
Phospho-SHP2 (Tyr542) 英文名稱： 磷酸化蛋白酪酸磷酸酶2抗體 0.1ml
Ephexin-1 英文名稱： 神經(jīng)細胞鳥苷酸置換因子NGEF抗體 0.2ml
細胞表面趨化因子受體10抗體 Anti-CCR-10 0.1ml
Anti-V5 tag/HRP 辣根過氧化物酶標記V5 tag標簽抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh phospho-HDAC8(Ser39) 磷酸化組蛋白去乙?；?抗體 規(guī)格 0.1ml
CD7 CD7抗原Multi-class antibodies規(guī)格： 0.5mg
人少突膠質(zhì)細胞
GIST 人細胞
TNFRSF13C Others Mouse 小鼠 BAFFR / TNFRSF13C / CD268 人細胞裂解液 (陽性對照)
CL-0434SC-1(小鼠胚胎細胞)5×106cells/瓶×2
人平滑肌細胞RNAHCSMC miRNA5 μg
人胚肺細胞VA13細胞;WI-38 VA13亞系2RA 人直平滑肌細胞完全培養(yǎng)基 100mL
CL-0194RF/6A(猴視網(wǎng)膜內(nèi)皮細胞)5×106cells/瓶×2
TCN2 Others Human 人 TCN2 / anscobalamin-II CHO細胞裂解液 (陽性對照)
人脈絡叢上皮細胞cDNAHCPEpiC cDNA
豬小腸上皮細胞;ZYM-DIEC02 成細胞，Saos-2細胞 RuCa細胞，小鼠細胞株
人胚腎細胞-F克隆;293F
HA Others H5N1 甲型 H5N1 (A/goose/Guiyang/337/2006) 血凝素HA1 (Hemagglutinin) 人細胞裂解液 (陽性對照)
RhoA抗體費用 人少突膠質(zhì)細胞
GIST 人細胞
TNFRSF13C Others Mouse 小鼠 BAFFR / TNFRSF13C / CD268 人細胞裂解液 (陽性對照)
CL-0434SC-1(小鼠胚胎細胞)5×106cells/瓶×2
人平滑肌細胞RNAHCSMC miRNA5 μg
人胚肺細胞VA13細胞;WI-38 VA13亞系2RA 人直平滑肌細胞完全培養(yǎng)基 100mL
