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| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS10860 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 |
| CAS編號(hào) | |
| 抗體名 | Anti-phospho-Proteasome 20S beta 7(Ser214) |
| 克隆性 | |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human Proteasome 20S beta 7 around the phosphorylation site of Ser214 |
產(chǎn)品訂購(gòu)信息：
英文名稱 Anti-phospho-Proteasome 20S beta 7(Ser214)
中文名稱 磷酸化蛋白酶體PSMβ7抗體說明書
別 名 Proteasome 20S beta 7 (phospho S214); Proteasome 20S beta 7 (phospho Ser214); p-Proteasome 20S beta 7 (S214); p-Proteasome 20S beta 7 (Ser214); 20S Proteasome β7; Macropain chain Z; Multicatalytic endopeptidase complex chain Z; Proteasome (prosome macropain) subunit beta type 7; Proteasome beta 7 subunit; Proteasome catalytic subunit 2; Proteasome subunit alpha; Proteasome subunit beta 7; Proteasome subunit beta type-7; Proteasome subunit Z; PSB7_HUMAN; PSMB7; PUP1.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep
產(chǎn)品類型 一抗 磷酸化抗體
研究領(lǐng)域 細(xì)胞生物 細(xì)胞周期蛋白 細(xì)胞分化
蛋白分子量 predicted molecular weight: 25kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Proteasome 20S beta 7 around the phosphorylation site of Ser214
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
磷酸化蛋白酶體PSMβ7抗體說明書 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.The proteasome represents a large protein complex that exists inside all eukaryotes and archaea, and in some bacteria. The main function of proteasomes is to degrade unnecessary or damaged proteins by proteolysis. The most common form of the proteasome, known as the 26S Proteasome, contains one 20S Proteasome core particle structure and two 19S regulatory caps. The 20S Proteasome core is hollow and forms an enclosed cavity, where proteins are degraded, as well as openings at the two ends to allow the target protein to enter. The 20S Proteasome core particle contains many subunits, depending on the organism.
Function : The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
Subunit : The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic equivalent immune-specific subunit PSMB10. Interacts with -1 TAT protein.
Subcellular Location : Cytoplasm. Nucleus.
Tissue Specificity : Expressed at a low level in colonic mucosa. Up-regulated in colorectal cancer tissues.
Similarity : Belongs to the peptidase T1B family.
Database links : UniProtKB/Swiss-Prot: Q99436.1
Anti-VAMP-3/FITC 熒光素標(biāo)記囊泡相關(guān)膜蛋白3抗體IgGMulti-class antibodies規(guī)格： 0.2ml
CD166/ALCAM(activated leukocyte celladhesion molecule) 活化白細(xì)胞粘附分子抗原Multi-class antibodies規(guī)格： 0.5mg
巨噬細(xì)胞來源的趨化因子抗體 Anti-CCL22/MDC 0.1ml
SPNS1 英文名稱： SPNS1抗體 0.2ml
EPS15 英文名稱： 胞吞調(diào)控蛋白Eps15抗體 0.2ml
Rhesus antibody Rh Phospho-HER4 (Tyr1188) 磷酸化HER4抗體 規(guī)格 0.1ml
CD166/ALCAM(activated leukocyte celladhesion molecule) 活化白細(xì)胞粘附分子抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-Melamine/HRP 辣根過氧化物酶標(biāo)記三聚氰胺單克隆抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-Mouse IgA Whole serum 兔抗小鼠IgA抗血清Multi-class antibodies規(guī)格： 1ml
白介素6抗體 Anti-IL-6 mouse、rat 0.1ml
Rabbit anti-Dog IgG whole serum 兔抗狗IgG抗血清 1ml
FZR1 英文名稱： 細(xì)胞分裂周期樣蛋白20抗體 0.2ml
Rhesus antibody Rh Phospho-Progesterone Receptor(Ser190) 磷酸化孕激素受體抗體 規(guī)格 0.1ml
Anti-Mouse IgA Whole serum 兔抗小鼠IgA抗血清Multi-class antibodies規(guī)格： 1ml
TLR-9/CD289(Human Toll-like receptor 9) ELISA kit 人Toll樣受體9Multi-class antibodies規(guī)格： 48T
Anti-Follistatin 卵泡抑素抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh Mouse Anti-human IgM/APC APC標(biāo)記的小鼠抗人IgM 規(guī)格 0.1ml
NON(Human nonmethylated oligonucleotide0 ELISA Kit 人非甲基化寡核苷酸 96T
SLITRK6 英文名稱： 神經(jīng)突觸相關(guān)蛋白SLITRK6抗體 0.2ml
CSMD3 英文名稱： CSMD3蛋白抗體 0.2ml
Anti-Follistatin 卵泡抑素抗體Multi-class antibodies規(guī)格： 0.2ml
Egg-YolkSaltAgarBase
EC-MUG培養(yǎng)基 100(g) incubation media EC-MUG培養(yǎng)基 100(g)
R2A瓊脂 R-2A Agar 250克 飲用水中導(dǎo)生細(xì)菌分離培養(yǎng)
DNA酶甲基綠瓊脂基礎(chǔ)于彎曲桿菌的DNA酶水解試驗(yàn)(GB標(biāo)準(zhǔn))incubationmediaDNA酶甲基綠瓊脂基礎(chǔ)于彎曲桿菌的DNA酶水解試驗(yàn)(GB標(biāo)準(zhǔn))
O/FMedium
梭菌強(qiáng)化培養(yǎng)基250g用于梭菌的增菌培養(yǎng)和計(jì)數(shù)
氯化鎂孔雀綠肉湯(MM，RV Rappaport-Vassiliadis Mdeium 用于沙門氏菌的選擇性增菌培養(yǎng)(GB標(biāo)準(zhǔn))
麥芽浸粉 Malt extract 250克 BR
改良CCDA培養(yǎng)基250g/瓶用于彎曲菌選擇性分離，每培養(yǎng)基中添加07cubationmedia改良CCDA培養(yǎng)基250g/瓶用于彎曲菌選擇性分離，每培養(yǎng)基中添加0711
緩沖MUG瓊脂 100g 用于濾膜MUG法檢測(cè)食品中大腸桿菌數(shù)(SN/T1059.2)
磷酸化蛋白酶體PSMβ7抗體說明書 尿素瓊脂培養(yǎng)基基礎(chǔ) 規(guī)格： 250g 用途： 用于鑒別腸道素酶試驗(yàn) (GB4789.4－2010，《中華人民共和國(guó)藥典》2010年版，SN0170-92和GB130...
SS瓊脂培養(yǎng)基 規(guī)格： 250g 用途： 用于沙門氏菌和某些志賀氏菌的選擇性分離培養(yǎng)(GB/T4789.28－2003 中4.22, GB/T4789.4－2003 和SN...
HE瓊脂培養(yǎng)基 規(guī)格： 250g 用途： 用于腸道致病菌特別是沙門氏菌和志賀氏菌的選擇性分離培養(yǎng)(GB 4789.4-2010和SN/T2206.1-2008)。
膽硫乳瓊脂培養(yǎng)基(DHL) 規(guī)格： 250g 用途： 用于腸道致病菌特別是沙門氏菌和志賀氏菌的選擇性分離培養(yǎng)。(《中華人民共和國(guó)藥典》2010年版)
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 磷酸化蛋白酶體PSMβ7抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及*基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定：
1）磷酸化蛋白酶體PSMβ7抗體說明書 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
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