|
| 產(chǎn)地 | 進口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS10809 |
| 應用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-Phospho-p73(Tyr99) |
| 克隆性 | |
| 靶點 | 詳見說明書 |
| 適應物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human p73 around the phosphorylation site of Tyr99 [SP(p-Y)AQ] |
技術外包服務:
★磷酸化P73抑制基因抗體費用 分子生物學：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學：基因合成、引物合成、基因測序、載體構建等
★蛋白工程：原核、哺乳動物蛋白表達系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細胞工程：細胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復、克隆形成)、細胞培養(yǎng)、細胞膜制備、穩(wěn)定細胞株構建、細胞RNAi技術等等。
實驗流程：
磷酸化P73抑制基因抗體費用 英文名稱 Anti-Phospho-p73(Tyr99)
中文名稱 磷酸化P73抑制基因抗體費用
別 名 p73(Phospho-Tyr99); TP73(phospho Y99); p53 like transcription factor; p53 Related Protein; p73; TP 73; TP73; Tumor Protein p73; P73_HUMAN.
產(chǎn)品屬性：
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應 Human, Mouse, Rat
產(chǎn)品類型 一抗 磷酸化抗體
研究領域 免疫學 信號轉導 細胞凋亡 轉錄調(diào)節(jié)因子 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 70kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human p73 around the phosphorylation site of Tyr99 [SP(p-Y)AQ]
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
磷酸化P73抑制基因抗體費用 產(chǎn)品應用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 P73 protein is a structural and functional homologue of p53, a tumor suppressor gene. In this study, The p73 protein, p19ras, by the yeast two-hybrid screening method. Alternative splicing of the proto-oncogene H-ras pre-mRNA has led to two distinct transcripts, Ras proteins are known to be small membrane-localized guanine nucleotide-binding proteins. However, unlike other Ras proteins, p19ras is localized in the nucleus and the cytosol and its interaction with P73 protein occurred exclusively in the nucleus. Oncogenic MDM2 (mouse double minutes 2) is a known repressor of p73 transcriptional activity. In this study, when p19ras was bound to MDM2, it further inhibited the association of MDM2 to the p73 protein. Therefore, this study presents a novel pathway of Ras signaling that occurs in the nucleus, involving p19ras and p73.
Function : Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. [COFACTOR] Binds 1 zinc ion per subunit
Subunit : Found in a complex with p53/TP53 and CABLES1. The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Interacts with HECW2. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9. Isoform Beta interacts with WWOX. Interacts (via SAM domain) with FBXO45 (via B30.2/SPRY domain). Interacts with YAP1 (phosphorylated form). Interacts with HCK (via SH3 domain); this inhibits TP73 activity and degradation.
Subcellular Location : Nucleus. Cytoplasm. Note=Accumulates in the nucleus in response to DNA damage.
Post-translational modifications : Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function.
Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
Polyubiquitinated by RCHY1/PIRH2; leading to its degradation by the proteasome.
Similarity : Belongs to the p53 family.
Contains 1 SAM (sterile alpha motif) domain.
Database links : UniProtKB/Swiss-Prot: O15350.1 (human)
UniProtKB/Swiss-Prot: Q9JJP2.1 (mouse)
P73是p53基因家族的第一個成員p53基因是一個經(jīng)典的基因,與p53在結構和功能方面具有很高的相似性。
全、新、優(yōu)、品、好四大特點：
磷酸化P73抑制基因抗體費用 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標準品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細胞等，基本上各種科研所需產(chǎn)品在我司都能找到。
新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。
優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。
好：我公司具有優(yōu)質(zhì)的技術團隊，產(chǎn)品一旦售出，實驗過程中遇到困難可提供在線技術咨詢。使您使用產(chǎn)品時沒有任何的后顧之憂。
正在熱銷的相關產(chǎn)品：
Anti-P/FITC 熒光素標記Raf激酶抑制蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml
GLP-2(Glucagon-like peptide-2) 胰素樣肽-2蛋白Multi-class antibodies規(guī)格： 0.5mg
蛋白裂解酶抗體 Anti-Matriptase 0.2ml
SOX9 英文名稱： 轉錄因子SOX9蛋白抗體 0.1ml
FAM134C 英文名稱： FAM134C蛋白抗體 0.2ml
Rhesus antibody Rh phospho-PIK3R1(Tyr556) 磷酸化磷脂酰肌醇激酶抗體 規(guī)格 0.1ml
GLP-2(Glucagon-like peptide-2) 胰素樣肽-2蛋白Multi-class antibodies規(guī)格： 0.5mg
Anti-MMP-17/FITC 熒光素標記基質(zhì)金屬蛋白酶-17抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-TSHR(CT)/FITC 熒光素標記促甲狀腺素受體抗體(C端)IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh A2BP1/Fox1 蛋白2結合蛋白1抗體 規(guī)格 0.2ml
Mouse Anti-Bov IgG/APC APC標記的小鼠抗IgG 0.1ml
GABARE 英文名稱： G基受體ε/GABAA Rε抗體 0.2ml
Rhesus antibody Rh PTPN7/HePTP 非受體型蛋白酪氨酸磷酸酶7抗體 規(guī)格 0.2ml
Anti-TSHR(CT)/FITC 熒光素標記促甲狀腺素受體抗體(C端)IgGMulti-class antibodies規(guī)格： 0.2ml
Anti- Alpha-IFN-b (humen) 抗大鼠α干擾素受體Multi-class antibodies規(guī)格： 48T
Anti-Dnmt3a DNA甲基轉移酶-3α抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh NCoR1 核受體輔助抑制因子1抗體 規(guī)格 0.1ml
Human Anti-Thrombin III ,AT- III ELISA Kit 人抗凝血酶Ⅲ抗體 96T
SIRT1 英文名稱： 沉默調(diào)節(jié)蛋白1抗體 0.1ml
Calretinin 英文名稱： 鈣結合蛋白抗體 0.1ml
Anti-Dnmt3a DNA甲基轉移酶-3α抗體Multi-class antibodies規(guī)格： 0.2ml
BacterialL-growingAgar
DEV dextrose broth DEV葡萄糖肉湯 1.10686.0500 MERCK默克 incubation media DEV dextrose broth DEV葡萄糖肉湯 1.10686.0500 MERCK默克
改良月桂基鹽胰蛋白月示肉湯(MLST) 250g 用于奶粉中阪崎腸桿菌的檢驗的增菌和分子生物學檢驗方法(PCR)選擇性增菌
兔骨髓間質(zhì)干細胞成脂誘導分化培養(yǎng)基Rabbitbonemarrowmesenchymalstemcellsintotheculturemediuminduceddifferentiationoffat
LM-137瓊脂 250g 用于濾膜法單增李氏菌選擇性分離(NEOGEN標準)
四酸煌綠增菌液基礎(TTB)2303g用于沙門氏菌選擇性增菌培養(yǎng)(《中華人民共和國藥典》20。
毛培養(yǎng)基基礎 (CM0161) Oxoid incubation media 毛培養(yǎng)基基礎 (CM0161) Oxoid
熱帶假絲酵母 產(chǎn)生蛋白酶(腐乳 菌) 支/瓶
TGE培養(yǎng)基250g用于細菌總數(shù)測定
SoybeanCaseinDigestMedium
磷酸化P73抑制基因抗體費用 營養(yǎng)肉湯 規(guī)格： 250g 用途： 一般細菌培養(yǎng)、轉種、復壯、增菌等，也可用于消毒效果測定。(GB)
溴紫葡萄糖蛋白胨水培養(yǎng)基 規(guī)格： 250g 用途： 用于壓力蒸汽滅菌效果的測定。(GB)
0.5%葡萄糖肉湯培養(yǎng)基 規(guī)格： 250g 用途： 用于環(huán)氧乙酸消毒和電離輻射消毒過程監(jiān)測指示菌的培養(yǎng)。(枯草桿菌黑色變種芽孢及短小桿菌芽孢) (...
無菌試驗用真菌培養(yǎng)基 規(guī)格： 250g 用途： 用于真菌殺滅試驗。（WS）
