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| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS10786 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-POMT1 |
| 克隆性 | |
| 靶點(diǎn) | 詳見(jiàn)說(shuō)明書(shū) |
| 適應(yīng)物種 | 詳見(jiàn)說(shuō)明書(shū) |
| 形態(tài) | 詳見(jiàn)說(shuō)明書(shū) |
| 宿主 | 詳見(jiàn)說(shuō)明書(shū) |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書(shū) |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human POMT1 |
產(chǎn)品訂購(gòu)信息：
英文名稱(chēng) Anti-POMT1
中文名稱(chēng) 蛋白甘露糖基轉(zhuǎn)移酶1抗體規(guī)格
別 名 POMT1_HUMAN; Protein O-mannosyl-transferase 1; Dolichyl-phosphate-mannose--protein mannosyltransferase 1.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來(lái)源 Rabbit
克隆類(lèi)型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow
產(chǎn)品類(lèi)型 一抗
研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 糖蛋白
蛋白分子量 predicted molecular weight: 85kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human POMT1
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
蛋白甘露糖基轉(zhuǎn)移酶1抗體規(guī)格 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 POMT1 (Protein O mannosyl transferase 1) is a multipass membrane protein that is found in the endoplasmic reticulum. POMT1 catalyses the transfer of mannosyl residues to the hydroxyl groups of serine or threonine residues. Enzymatic activity is dependent on co expression of POMT1 with POMT2. Defects in the POMT1 gene are associated with Walker-Warburg syndrome (WWS), a congential muscular dystrophy that is associated with mental retardation and is usually lethal within the first few months of life. Other defects in the POMT1 gene result in limb girdle muscular dystrophy type 2K (LGMD2K), which is associated with mild mental retardation. Studies in Drosophila suggest that mutation of POMT1 alters the efficacy of synaptic transmission and a change in subunit composition of post synaptic glutamate receptors at the neuromuscular junction. Missense mutations in POMT1 have been associated with glioneuronal and glial brain tumours.
Function : Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient.
Subunit : Interacts with POMT2 (Probable).
Subcellular Location : Endoplasmic reticulum membrane; Multi-pass membrane protein.
DISEASE : Muscular dystrophy-dystroglycanopathy congenital with mental retardation B1 (MDDGB1) [MIM:613155]: An autosomal recessive disorder characterized by congenital muscular dystrophy associated with mental retardation and mild structural brain abnormalities. Note=The disease is caused by mutations affecting the gene represented in this entry.
Muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies A1 (MDDGA1) [MIM:236670]: An autosomal recessive disorder characterized by congenital muscular dystrophy associated with cobblestone lissencephaly and other brain anomalies, eye malformations, profound mental retardation, and death usually in the first years of life. Included diseases are the more severe Walker-Warburg syndrome and the slightly less severe muscle-eye-brain disease. Note=The disease is caused by mutations affecting the gene represented in this entry.
Muscular dystrophy-dystroglycanopathy limb-girdle C1 (MDDGC1) [MIM:609308]: An autosomal recessive degenerative myopathy associated with mild mental retardation without any obvious structural brain abnormality. An abnormal alpha-dystroglycan pattern in observed in the muscle. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity : Belongs to the glycosyltransferase 39 family.
Contains 3 MIR domains.
Database links : UniProtKB/Swiss-Prot: Q9Y6A1.3
Anti-ATP2c1/Ca++ ATPase/FITC 熒光素標(biāo)記鈣離子ATP酶通道蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-GADD153/FITC 熒光素標(biāo)記生長(zhǎng)抑制DNA損傷基因153抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh CLPTM1L/CRR9 順氯氨鉑耐藥相關(guān)蛋白9抗體 規(guī)格 0.2ml
midnolin isoform Protein 2 中腦核仁蛋白2(抗原) 0.5mg
IGLK 英文名稱(chēng)： IV型己糖激酶抗體 0.2ml
Rhesus antibody Rh TRPM1 瞬時(shí)受體電位離子通道蛋白1抗體(M亞家族) 規(guī)格 0.2ml
Anti-GADD153/FITC 熒光素標(biāo)記生長(zhǎng)抑制DNA損傷基因153抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-IKB alpha(phospho S32 + S36)/FITC 熒光素標(biāo)記磷酸化KB抑制蛋白α抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-Phospho-PLK1 (Thr210)/FITC 熒光素標(biāo)記磷酸化絲/蘇氨酸蛋白激酶Plk1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh ARHGAP20 Rho GTP酶激活蛋白20抗體 規(guī)格 0.2ml
Rabbit Anti-Mink IgG/PE-CY5 PE-CY5標(biāo)記的兔抗水貂IgG 0.1ml
GADL1 英文名稱(chēng)： 谷酸脫羧酶樣蛋白1抗體 0.2ml
Rhesus antibody Rh Rabbit Anti-Mink IgG/RBITC 羅丹明標(biāo)記的兔抗水貂IgG 規(guī)格 0.1ml
Anti-Phospho-PLK1 (Thr210)/FITC 熒光素標(biāo)記磷酸化絲/蘇氨酸蛋白激酶Plk1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
NT-4 ELISA Kit 大鼠神經(jīng)營(yíng)養(yǎng)因子4Multi-class antibodies規(guī)格： 48T
Anti-B7-H1/PDL1/CD274 /FITC 熒光素標(biāo)記程序性死亡配體1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh EURL/C21orf91 21號(hào)染色體開(kāi)放閱讀框91抗體 規(guī)格 0.2ml
PF-4/CXCL4 (Rabbit Platelet Factor 4) ELISA Kit 兔子血小板因子4 96T
Phospho-MKK3(Ser218) + MKK6(Ser207) 英文名稱(chēng)： 磷酸化原活化蛋白激酶MKK3/6抗體 0.1ml
APAF1(CT) 英文名稱(chēng)： 凋亡蛋白活性因子-1抗體(C端) 0.1ml
Anti-B7-H1/PDL1/CD274 /FITC 熒光素標(biāo)記程序性死亡配體1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
615小鼠前瘤株;Fc
ART4 Others Cynomolgus 食蟹猴 ART4 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
CM-M029小鼠腸平滑肌細(xì)胞完全培養(yǎng)基100mL
TE-11細(xì)胞，人細(xì)胞 人細(xì)胞,TJ905細(xì)胞 犬腎細(xì)胞;MDCK(NBL-2)
Calu-3(人) 5×106cells/瓶×2
CD40 Others Human 人 CD40 / TNFRSF5 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
615小鼠前瘤株;Fc
ART4 Others Cynomolgus 食蟹猴 ART4 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
CM-M029小鼠腸平滑肌細(xì)胞完全培養(yǎng)基100mL
TE-11細(xì)胞，人細(xì)胞 人細(xì)胞,TJ905細(xì)胞 犬腎細(xì)胞;MDCK(NBL-2)
Calu-3(人) 5×106cells/瓶×2
CD40 Others Human 人 CD40 / TNFRSF5 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
蛋白甘露糖基轉(zhuǎn)移酶1抗體規(guī)格 人表皮角質(zhì)細(xì)胞-(HEK-a) ( 5×105 )
人肺粘液上皮樣癌細(xì)胞;NCI-H292
大鼠骨髓間充質(zhì)干細(xì)胞(BMSCs)
人細(xì)胞;CNE-2Z 大鼠腎上皮細(xì)胞完全培養(yǎng)基 100mL
NG108-15 大鼠小鼠雜合神經(jīng)細(xì)胞
IL2RA Others Mouse 小鼠 IL2RA / CD25 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 蛋白甘露糖基轉(zhuǎn)移酶1抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；
3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴(lài)氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及*基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定：
1）蛋白甘露糖基轉(zhuǎn)移酶1抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線(xiàn),計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
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