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| 產(chǎn)地 | 進(jìn)口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS10133 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-Prion protein PrP |
| 克隆性 | |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human Major prion protein |
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 朊/感染性蛋白抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時,或位于酶的催化位點(diǎn)時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及*基,也可與糖基共價結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。
產(chǎn)品訂購信息：
英文名稱 Anti-Prion protein PrP
中文名稱 朊/感染性蛋白抗體費(fèi)用
別 名 AltPrP; ASCR; atal familial insomnia; CD230; CD230 antigen; CJD; Creutzfeld Jakob disease; Gerstmann-Strausler-Scheinker syndrome; GSS; KURU; Major prion protein; MGC26679; p27 30; PRIO_HUMAN; Prion protein; Prion related protein; PRIP; Prni; Prnp; PrP; PrP27 30; PrP27-30; PrP33 35C; PrP33-35C; PrPC; PrPSc; Sinc.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Cow, Horse, Sheep
產(chǎn)品類型 一抗
研究領(lǐng)域 免疫學(xué) 神經(jīng)生物學(xué) 干細(xì)胞 細(xì)胞凋亡 細(xì)胞表面分子 Alzheimer's
蛋白分子量 predicted molecular weight: 23kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Major prion protein
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
朊/感染性蛋白抗體費(fèi)用 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012].
Function : May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparin sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains.
Subunit : Monomer and homodimer. Has a tendency to aggregate into amyloid fibrils containing a cross-beta spine, formed by a steric zipper of superposed beta-strands. Soluble oligomers may represent an intermediate stage on the path to fibril formation. Copper binding may promote oligomerization. Interacts with GRB2, APP, ERI3/PRNPIP and SYN1. Mislocalized cytosolically exposed PrP interacts with MGRN1; this interaction alters MGRN1 subcellular location and causes lysosomal enlargement. Interacts with KIAA1191.
Subcellular Location : Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus. Note=Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of CU(2+), to vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis.
Post-translational modifications : The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion.
Isoform 2 is sumoylated with SUMO1.
DISEASE : Note=PrP is found in high quantity in the brain of humans and animals infected with neurodegenerative diseases known as transmissible spongiform encephalopathies or prion diseases, like: Creutzfeldt-Jakob disease (CJD), fatal familial insomnia (FFI), Gerstmann-Straussler disease (GSD), Huntington disease-like type 1 (HDL1) and kuru in humans; scrapie in sheep and goat; bovine spongiform encephalopathy (BSE) in cattle; transmissible mink encephalopathy (TME); chronic wasting disease (CWD) of mule deer and elk; feline spongiform encephalopathy (FSE) in cats and exotic ungulate encephalopathy (EUE) in nyala and greater kudu. The prion diseases illustrate three manifestations of CNS degeneration: (1) infectious (2) sporadic and (3) dominantly inherited forms. TME, CWD, BSE, FSE, EUE are all thought to occur after consumption of prion-infected foodstuffs.
Defects in PRNP are the cause of Creutzfeldt-Jakob disease (CJD) [MIM:123400]. CJD occurs primarily as a sporadic disorder (1 per million), while 10-15% are familial. Accidental transmission of CJD to humans appears to be iatrogenic (contaminated human growth hormone (HGH), corneal transplantation, electroencephalographic electrode implantation, etc.). Epidemiologic studies have failed to implicate the ingestion of infected annimal meat in the pathogenesis of CJD in human. The triad of microscopic features that characterize the prion diseases consists of (1) spongiform degeneration of neurons, (2) severe astrocytic gliosis that often appears to be out of proportion to the degree of nerve cell loss, and (3) amyloid plaque formation. CJD is characterized by progressive dementia and myoclonic seizures, affecting adults in mid-life. Some patients present sleep disorders, abnormalities of high cortical function, cerebellar and corticospinal disturbances. The disease ends in death after a 3-12 months illness.
Defects in PRNP are the cause of fatal familial insomnia (FFI) [MIM:600072]. FFI is an autosomal dominant disorder and is characterized by neuronal degeneration limited to selected thalamic nuclei and progressive insomnia.
Defects in PRNP are the cause of Gerstmann-Straussler disease (GSD) [MIM:137440]. GSD is a heterogeneous disorder and was defined as a spinocerebellar ataxia with dementia and plaquelike deposits. GSD incidence is less than 2 per 100 million live births.
Defects in PRNP are the cause of Huntington disease-like type 1 (HDL1) [MIM:603218]. HDL1 is an autosomal dominant, early onset neurodegenerative disorder with prominent psychiatric features.
Defects in PRNP are the cause of kuru (KURU) [MIM:245300]. Kuru is transmitted during ritualistic cannibalism, among natives of the New Guinea highlands. Patients exhibit various movement disorders like cerebellar abnormalities, rigidity of the limbs, and clonus. Emotional lability is present, and dementia is conspicuously absent. Death usually occurs from 3 to 12 month after onset.
Defects in PRNP are the cause of spongiform encephalopathy with neuropsychiatric features (SENF) [MIM:606688]; an autosomal dominant presenile dementia with a rapidly progressive and protracted clinical course. The dementia was characterized clinically by frontotemporal features, including early personality changes. Some patients had memory loss, several showed aggressiveness, hyperorality and verbal stereotypy, others had parkinsonian symptoms.
Similarity : Belongs to the prion family.
Database links : UniProtKB/Swiss-Prot: P04156.1 human
UniProtKB/Swiss-Prot:P10279 Bovine
抗體的鑒定：
1）朊/感染性蛋白抗體費(fèi)用 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力。抗體的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
FA ELISA Kit 大鼠葉酸Multi-class antibodies規(guī)格： 48T
Anti-Tubb3 微管蛋白β3抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh GDPD5 甘油磷酸二酯酶磷酸結(jié)構(gòu)域5抗體 規(guī)格 0.2ml
TSGF ELISA Kit 大鼠特異生長因子/相關(guān)因子 96T
Nm23-H2 英文名稱： 抑制基因抗體 0.1ml
AQP0 英文名稱： 水通道蛋白0抗體 0.1ml
Anti-Tubb3 微管蛋白β3抗體Multi-class antibodies規(guī)格： 0.2ml
GHR (growth hormone receptor) 生長激素受體(抗原)Multi-class antibodies規(guī)格： 0.5mg
Anti-CD14 內(nèi)毒素受體抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh PARL 骨骼肌細(xì)胞線粒體膜蛋白PARL抗體 規(guī)格 0.2ml
AHSG Kit Mouse 小鼠 Fetuin-A / AHSG ELISA配對抗體 ELISA
TREML2 英文名稱： 髓系細(xì)胞觸發(fā)受體樣轉(zhuǎn)錄因子2抗體 0.2ml
cellulase 英文名稱： 纖維素酶抗體 1ml
Anti-CD14 內(nèi)毒素受體抗體Multi-class antibodies規(guī)格： 0.1ml
GRH ELISA Kit 大鼠促生長激素釋放激素Multi-class antibodies規(guī)格： 48T
Anti-AT1/FITC 熒光素標(biāo)記緊張素I抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh FAK/PTK2 粘著斑激酶抗體 規(guī)格 0.1ml
TPO-Ab(Mouse anti-Thyroid-Peroxidase antibody) ELISA Kit 小鼠抗甲狀腺過氧化物酶抗體 96T
Mesothelin 英文名稱： 間皮素抗體 0.1ml
ADH6 英文名稱： 乙醇脫氫酶6抗體 0.2ml
Anti-AT1/FITC 熒光素標(biāo)記緊張素I抗體IgGMulti-class antibodies規(guī)格： 0.2ml
人臍帶血單個核細(xì)胞
NCI-H460 人大細(xì)胞細(xì)胞
MS4A1 Others Ferret 雪貂 CD20 / MS4A-1 人細(xì)胞裂解液 (陽性對照)
COS-7L, 非洲綠猴腎成纖維細(xì)胞
人肝細(xì)胞;HL-7702[L-02]
人胚腎細(xì)胞;2V6.11 人*上皮細(xì)胞完全培養(yǎng)基 100mL
CL-0220STO(小鼠胚成纖維細(xì)胞)5×106cells/瓶×2
CD53 Others Cynomolgus 食蟹猴 CD53 (aa 107-181) 人細(xì)胞裂解液 (陽性對照)
人基質(zhì)細(xì)胞cDNAHHGMC cDNA
LCC9細(xì)胞，人癌抗雌激素耐藥株 草魚*細(xì)胞,CIK細(xì)胞 肝星形細(xì)胞Many types of cells包裝：5 × 105次方(1ml)
293FT(人胚腎細(xì)胞) 5×106cells/瓶×2
HPF Pellet 人團(tuán)塊 > 1 mio.cells 人海馬趾神經(jīng)細(xì)胞裂解物HN-h L
朊/感染性蛋白抗體費(fèi)用 人臍帶血單個核細(xì)胞
NCI-H460 人大細(xì)胞細(xì)胞
MS4A1 Others Ferret 雪貂 CD20 / MS4A-1 人細(xì)胞裂解液 (陽性對照)
COS-7L, 非洲綠猴腎成纖維細(xì)胞
人肝細(xì)胞;HL-7702[L-02]
人胚腎細(xì)胞;2V6.11 人*上皮細(xì)胞完全培養(yǎng)基 100mL
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