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| 產(chǎn)地 | 進(jìn)口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS10114 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-PRG2 |
| 克隆性 | |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human PRG2 |
產(chǎn)品訂購信息：
英文名稱 Anti-PRG2
中文名稱 嗜酸性粒細(xì)胞顆粒關(guān)鍵堿性蛋白抗體規(guī)格
別 名 BMPG; EMBP; Eosinophil granule major basic protein; Eosinophil major basic protein; MBP; MBP1; Natural killer cell activator; Pregnancy-associated major basic protein; PRG 2; PRG2; PRG2_HUMAN; Proteoglycan 2; Proteoglycan 2 bone marrow; Proteoglycan 2 preproprotein.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 免疫學(xué) 自然殺傷細(xì)胞 淋巴細(xì)胞
蛋白分子量 predicted molecular weight: 25kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human PRG2
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
嗜酸性粒細(xì)胞顆粒關(guān)鍵堿性蛋白抗體規(guī)格 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011].
Function : Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
Subunit : In pregnancy serum, the proform exists as a disulfide-linked 2:2 heterotetramer with PAPPA, as a disulfide-linked 2:2 heterotetramer with AGT, and as a complex (probably a 2:2:2 heterohexamer) with AGT and C3dg.
Subcellular Location : Bone marrow proteoglycan: Secreted. Note=The proform is secreted.
Tissue Specificity : High levels of the proform in placenta and pregnancy serum; in placenta, localized to X cells of septa and anchoring villi. Lower levels in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow and colon.
Post-translational modifications : Nitrated.
Similarity : Contains 1 C-type lectin domain.
Database links : NCBI Reference Sequence: NP_002719.3
UniProtKB/Swiss-Prot: P13727.2
Anti-Caspase-1/FITC 熒光素標(biāo)記天冬氨酸-胱氨酸特異性蛋白酶家族抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Anti-A/H2N2 /FITC 熒光素標(biāo)記兔抗甲2(H2N2)亞型抗體(甲2型)IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh CGR19 細(xì)胞生長(zhǎng)調(diào)控蛋白19抗體(環(huán)指蛋白197) 規(guī)格 0.2ml
Trop-2/Tpm2 peptide 原肌球蛋白抗原 0.5mg
intestinal FABP 英文名稱： 小腸型脂肪酸結(jié)合蛋白抗體 0.1ml
Rhesus antibody Rh TM9SF1 跨膜蛋白超家族9-1抗體 規(guī)格 0.1ml
Anti-A/H2N2 /FITC 熒光素標(biāo)記兔抗甲2(H2N2)亞型抗體(甲2型)IgGMulti-class antibodies規(guī)格： 0.2ml
TPH (Trptophan Hydroxylase) 色氨酸羥化酶(多肽)Multi-class antibodies規(guī)格： 0.5mg
Anti-Calpain 2 鈣激活的中性蛋白酶2抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh PDGFRA 血小板源性生長(zhǎng)因子受體A抗體(PDGFRα) 規(guī)格 0.1ml
Apolipoprotein D 濃縮液 0.1ml 進(jìn)口分裝
TRAIL 英文名稱： 壞死因子相關(guān)凋亡誘導(dǎo)配體抗體 0.1ml
CEA 英文名稱： 癌胚抗原抗體 0.1ml
Anti-Calpain 2 鈣激活的中性蛋白酶2抗體Multi-class antibodies規(guī)格： 0.1ml
OT/BGP ELISA Kit 大鼠骨鈣素/骨谷氨酸蛋白Multi-class antibodies規(guī)格： 48T
Anti-ATF4/FITC 熒光素標(biāo)記活化轉(zhuǎn)錄因子4抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh factor XIII 凝血因子13抗體(纖維蛋白穩(wěn)定因子) 規(guī)格 0.2ml
BGP(Osteocalcin/Bone Gla-protein)ELISA Kit 兔骨鈣素 ELISA試劑盒 96T
Mycobacterium tuberculosis Ag85B 英文名稱： 結(jié)核分枝桿菌抗體 0.2ml
ABCG2 英文名稱： 三磷酸腺苷結(jié)合轉(zhuǎn)運(yùn)蛋白G超家族成員2抗體 0.1ml
Anti-ATF4/FITC 熒光素標(biāo)記活化轉(zhuǎn)錄因子4抗體IgGMulti-class antibodies規(guī)格： 0.2ml
THPO Protein Cynomolgus 重組食蟹猴 Thrombopoietin / THPO / TPO 蛋白 (His 標(biāo)簽)
MPC-83 *腺泡細(xì)胞癌
CD33 Others Mouse 小鼠 CD33 / Siglec-3 人細(xì)胞裂解液 (陽性對(duì)照)
IMR-32人神經(jīng)母細(xì)胞瘤細(xì)胞 IMR-32 human neuroblastoma cells MEM培養(yǎng)基(GIBCO)+10%FBS
人細(xì)胞;SF126
人上皮細(xì)胞;MCF-10A 人平滑肌細(xì)胞完全培養(yǎng)基 100mL
CL-0257BC-009(人癌細(xì)胞)5×106cells/瓶×2
RP2 Others Human 人 XRP2 / RP2 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對(duì)照)
人成纖維細(xì)胞cDNAHPrF cDNA
C6細(xì)胞，腦細(xì)胞 人肺巨細(xì)胞癌細(xì)胞,HLAmP細(xì)胞 肺大動(dòng)脈平滑肌細(xì)胞Many types of cells包裝：5 × 105次方(1ml)
犬腎細(xì)胞;MDCK(NBL-2)
EM1 Others Human 人 EM1 人細(xì)胞裂解液 (陽性對(duì)照)
嗜酸性粒細(xì)胞顆粒關(guān)鍵堿性蛋白抗體規(guī)格 THPO Protein Cynomolgus 重組食蟹猴 Thrombopoietin / THPO / TPO 蛋白 (His 標(biāo)簽)
MPC-83 *腺泡細(xì)胞癌
CD33 Others Mouse 小鼠 CD33 / Siglec-3 人細(xì)胞裂解液 (陽性對(duì)照)
IMR-32人神經(jīng)母細(xì)胞瘤細(xì)胞 IMR-32 human neuroblastoma cells MEM培養(yǎng)基(GIBCO)+10%FBS
人細(xì)胞;SF126
人上皮細(xì)胞;MCF-10A 人平滑肌細(xì)胞完全培養(yǎng)基 100mL
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 嗜酸性粒細(xì)胞顆粒關(guān)鍵堿性蛋白抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及*基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定：
1）嗜酸性粒細(xì)胞顆粒關(guān)鍵堿性蛋白抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
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