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| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS10050 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-Oligophrenin 1 |
| 克隆性 | |
| 靶點(diǎn) | 詳見(jiàn)說(shuō)明書 |
| 適應(yīng)物種 | 詳見(jiàn)說(shuō)明書 |
| 形態(tài) | 詳見(jiàn)說(shuō)明書 |
| 宿主 | 詳見(jiàn)說(shuō)明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from huamn Oligophrenin 1 (418-465aa) |
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 精神發(fā)育遲滯相關(guān)蛋白Oligophrenin-1抗體規(guī)格 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；
3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及*基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。
產(chǎn)品訂購(gòu)信息：
英文名稱 Anti-Oligophrenin 1
中文名稱 精神發(fā)育遲滯相關(guān)蛋白Oligophrenin-1抗體規(guī)格
別 名 Oligophrenin1; Oligophrenin-1; OPHN1_HUMAN.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來(lái)源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Cow, Horse, Sheep
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) G蛋白信號(hào)
蛋白分子量 predicted molecular weight: 92kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from huamn Oligophrenin 1 (418-465aa)
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
精神發(fā)育遲滯相關(guān)蛋白Oligophrenin-1抗體規(guī)格 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Ras p21 can exist in either a physiologically quiescent GDP-binding state or a GTP-binding signal-emitting state (1,2). Interaction of Ras p21 with GTPase activating protein (GAP) can increase the rate of hydrolysis of Ras p21-bound GTP by as much as 1000-fold (3). In mitogenically activated and tyrosine kinase-transformed cells, Ras GAP forms a complex with a protein designated p190 (4). At its amino terminus, p190 contains sequence motifs characteristic of all known GTPases, whereas the carboxy terminus contains sequences similar to those found in the Bcr gene product, n-chimerin and Rho GAP, all of which exhibit intrinsic GAP activity (4,5). Oligophrenein-1 is an additional protein with GTPase activating activity. Oligophrenein-1 is a RhoGAP protein that stimulates GTP hydrolysis of Rho subfamily members and is involved in cell migration, morphogenesis and axon outgrowth (6).
Function : Stimulates GTP hydrolysis of members of the Rho family. Could activates GTPase targets that are known to affect cell migration and outgrowth of axons and dendrites.
Subunit : Interacts with HOMER1. Interacts with AMPA receptor complexes. Interacts with SH3GL2 (endophilin-A1)
Subcellular Location : Cell junction, synapse. Cell projection, axon. Cell projection, dendritic spine. Note=Present in both presynaptic and postsynaptic sites
Tissue Specificity : Expressed in brain.
DISEASE : Defects in OPHN1 are the cause of mental retardation X-linked OPHN1-related (MRXSO) [MIM:300486]; formerly designated MRX60. MRXSO is a syndromic mental retardation. Patients present mental retardation associated with cerebellar hypoplasia and distinctive facial dysmorphism.
Similarity : Contains 1 PH domain.
Contains 1 Rho-GAP domain.
Database links : UniProtKB/Swiss-Prot: O60890.1
抗體的鑒定：
1）精神發(fā)育遲滯相關(guān)蛋白Oligophrenin-1抗體規(guī)格 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
EM-2(Human endomorphin-2) ELISA Kit 人內(nèi)*肽-2Multi-class antibodies規(guī)格： 48T
Anti-Histone H2A 組蛋白H2AX抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh MMP-10 基質(zhì)金屬蛋白酶-10抗體 規(guī)格 0.1ml
TPO(Human Thyroid-Peroxidase) ELISA Kit 人甲狀腺過(guò)氧化物酶 96T
phospho-RUNX2(Ser451) 英文名稱： 磷酸化成骨特異性轉(zhuǎn)錄因子抗體 0.1ml
Complement C3dg fragment 英文名稱： 補(bǔ)體C3dg片段抗體 0.1ml
Anti-Histone H2A 組蛋白H2AX抗體Multi-class antibodies規(guī)格： 0.2ml
FGF-4 human 人成纖維生長(zhǎng)因子-4Multi-class antibodies規(guī)格： 48T
Anti-E Tag E Tag標(biāo)簽抗體Multi-class antibodies規(guī)格： 0.1ml
Rhesus antibody Rh NOX4/NADPH oxidase 4 NADPH氧化酶NOX家族的成員4抗體 規(guī)格 0.2ml
AQP-4(Human Aquaporin 4) ELISA Kit 人水通道蛋白4 96T
TNF-alpha 英文名稱： 壞死因子-α抗體 0.1ml
C6orf201 英文名稱： 6號(hào)染色體開(kāi)放閱讀框201抗體 0.2ml
Anti-E Tag E Tag標(biāo)簽抗體Multi-class antibodies規(guī)格： 0.1ml
Parvalbumin 微白蛋白/細(xì)小清蛋白抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-BADH2 BADH2抗體(水稻)Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh Phospho-5-Lipoxygenase(Ser663) 磷酸化5-脂氧合酶抗體 規(guī)格 0.1ml
Keratin19 濃縮液 0.1ml 進(jìn)口分裝
UBE2A 英文名稱： 泛素結(jié)合酶E2蛋白A抗體 0.2ml
CHST10 英文名稱： 碳水化合物磺基轉(zhuǎn)移酶10抗體 0.1ml
Anti-BADH2 BADH2抗體(水稻)Multi-class antibodies規(guī)格： 0.2ml
內(nèi)皮生長(zhǎng)因子
Hep G2 人細(xì)胞
ERBB3 Others Mouse 小鼠 HER3 / ErbB3 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
SW 982 [SW-982, SW982]人滑膜細(xì)胞 SW 982 [SW-982, SW982] human synovial sarcoma cells L-15(GIBCO)+10%FBS
SV40轉(zhuǎn)化的非洲綠猴腎細(xì)胞;COS-1
大鼠腹部脊髓神經(jīng)元(RVSCI)(1×106) HNE2, 人細(xì)胞系 Human
CL-0389NCI-H1688(人典型小細(xì)胞細(xì)胞)5×106cells/瓶×2
S100A4 Others Mouse 小鼠 S100A4 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
人無(wú)色素睫狀上皮細(xì)胞總RNAHNPCEpiC NA
大耳山羊肺細(xì)胞;LDG-1 大鼠主動(dòng)脈平滑肌細(xì)胞，A10細(xì)胞 BNL1ME A.7R.1細(xì)胞，BALB/C小鼠肝上皮細(xì)胞
人胚腎二倍體細(xì)胞;HEK-2
HA Others H1N1 甲型 H1N1 (A/New Caledonia/20/1999) 血凝素 (Hemagglutinin / HA) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
精神發(fā)育遲滯相關(guān)蛋白Oligophrenin-1抗體規(guī)格 內(nèi)皮生長(zhǎng)因子
Hep G2 人細(xì)胞
ERBB3 Others Mouse 小鼠 HER3 / ErbB3 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)
SW 982 [SW-982, SW982]人滑膜細(xì)胞 SW 982 [SW-982, SW982] human synovial sarcoma cells L-15(GIBCO)+10%FBS
SV40轉(zhuǎn)化的非洲綠猴腎細(xì)胞;COS-1
大鼠腹部脊髓神經(jīng)元(RVSCI)(1×106) HNE2, 人細(xì)胞系 Human
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