|
| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS9769 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-NOTCH1/activated Notch1 |
| 克隆性 | |
| 靶點(diǎn) | 詳見(jiàn)說(shuō)明書 |
| 適應(yīng)物種 | 詳見(jiàn)說(shuō)明書 |
| 形態(tài) | 詳見(jiàn)說(shuō)明書 |
| 宿主 | 詳見(jiàn)說(shuō)明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見(jiàn)說(shuō)明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human NOTCH1/activated Notch1 (1687-1730aa) |
中文名稱 胞內(nèi)活化的Notch1蛋白抗體費(fèi)用
英文名稱 Anti-NOTCH1/activated Notch1
別 名 hN1; Neurogenic locus Notch homolog protein 1; activated Notch 1; activated Notch-1;Notch homolog 1 translocation associated (Drosophila); NOTCH1; TAN1; Translocation-associated Notch protein TAN-1;
產(chǎn)品屬性：
胞內(nèi)活化的Notch1蛋白抗體費(fèi)用 濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來(lái)源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Sheep
產(chǎn)品類型 一抗
研究領(lǐng)域 心 細(xì)胞生物 發(fā)育生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 干細(xì)胞 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué)
蛋白分子量 predicted molecular weight: 86/89/271kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human NOTCH1/activated Notch1 (1687-1730aa)
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
胞內(nèi)活化的Notch1蛋白抗體費(fèi)用 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 The LIN-12/notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. To date, four notch homologs have been identified in mammals and have been designated Notch 1, Notch 2, Notch 3 and Notch 4. The notch genes are expressed in a variety of tissues in both the embryonic and adult organism, suggesting that the genes are involved in multiple signaling pathways. The notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for notch include Jagged1, Jagged2 and Delta. Jagged can activate notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. Jagged2 is thought to be involved in the development of various tissues whose development is dependent upon epithelial-mesenchymal interactions. Normal Delta expression is restricted to the adrenal gland and placenta. Delta expression has also been found in neuroendocrine tumors such as neuroblastomas and pheochromocytomas.
Function : Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. The protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands. Once the Notch extracellular domain interacts with a ligand, a protease called TACE (Tumor Necrosis Factor Alpha Converting Enzyme) cleaves the Notch protein just outside the membrane. This releases the extracellular portion of Notch, which continues to interact with the ligand. The ligand plus the Notch extracellular domain is then endocytosed by the ligand expressing cell. After this first cleavage, an enzyme called gamma-secretase cleaves the remaining part of the Notch protein just inside the inner leaflet of the cell membrane. This releases the intracellular portion of the Notch protein, which then moves to the nucleus and causes various genes to be expressed. There are many other proteins involved in the intracellular portion of the Notch signalling cascade.Subunit : Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiationSubcellular Location : Cell membrane; Single-pass type I membrane protein (By similarity).Notch 1 intracellular domain: Nucleus (By similarity). Note=Following proteolytical processing NICD is translocated to the nucleusTissue Specificity : In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.Post-translational modifications : Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).Phosphorylated (By similarity).O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch.Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN (By similarity). Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICDDISEASE : Defects in NOTCH1 are a cause of aortic valve disease 1 (AOVD1) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.Similarity : Belongs to the NOTCH family.Contains 5 ANK repeats.Contains 36 EGF-like domains.Contains 3 LNR (Lin/Notch) repeats.Database links : UniProtKB/Swiss-Prot: P46531.4
實(shí)驗(yàn)流程：
全、新、優(yōu)、品、好四大特點(diǎn)：
胞內(nèi)活化的Notch1蛋白抗體費(fèi)用 全：公司提供上萬(wàn)種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。
新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。
優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。
好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過(guò)程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒(méi)有任何的后顧之憂。
技術(shù)外包服務(wù):
★胞內(nèi)活化的Notch1蛋白抗體費(fèi)用 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等
★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。
正在熱銷的相關(guān)產(chǎn)品：
Anti-Phospho-delta Opioid Receptor (Ser363) /FITC 熒光素標(biāo)記磷酸化D型受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rabbit Anti-mouse IgG/HRP 辣根過(guò)氧化物酶標(biāo)記兔抗小鼠IgGMulti-class antibodies規(guī)格： 0.1ml
c-erbB-2抗體 Anti-C-erbB-2 0.1ml
Rabbit Anti-horse IgM/Cy3 Cy3標(biāo)記的兔抗馬IgM 0.1ml
FABPB 英文名稱： 腦型脂肪酸結(jié)合蛋白抗體 0.2ml
Rhesus antibody Rh PKMYT1 髓鞘轉(zhuǎn)錄因子1激酶抗體 規(guī)格 0.1ml
Rabbit Anti-mouse IgG/HRP 辣根過(guò)氧化物酶標(biāo)記兔抗小鼠IgGMulti-class antibodies規(guī)格： 0.1ml
LN (laminin) 層粘連蛋白(多肽抗原)Multi-class antibodies規(guī)格： 0.5mg
Anti-ALK/CD246 間變型激酶抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh phospho-c-Abl(Tyr251) 磷酸化非受體酪氨酸激酶c-Abl抗體 規(guī)格 0.1ml
低背景抗體稀釋液 10ml/100ml pH 7.6
VWCE 英文名稱： VWCE抗體 0.2ml
phospho-DAXX (Ser495) 英文名稱： 磷酸化Fas相關(guān)死亡結(jié)構(gòu)域蛋白抗體 0.1ml
Anti-ALK/CD246 間變型激酶抗體Multi-class antibodies規(guī)格： 0.2ml
SEPT6 英文名稱： 細(xì)胞分化蛋白SEPT6抗體 0.2ml
EML3 英文名稱： 微管相關(guān)蛋白樣蛋白3抗體 0.2ml
組織蛋白酶K抗體 Anti-cath-K/CK 0.1ml
Anti-UG/FITC 熒光素標(biāo)記子宮珠蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh Phospho-Histone H3 (Thr3) 磷酸化組蛋白H3抗體 規(guī)格 0.1ml
CCR-7(CC chemokine receptor 7) CC趨化因子受體7(多肽)Multi-class antibodies規(guī)格： 0.5mg
HE瓊脂(HE)250g用于沙門氏菌的選擇性分離培養(yǎng)(GB標(biāo)準(zhǔn))
JMMediumBase
噻孢霉素 A 1.25μg/支*5 添加于100ml HB0121中
RNS培養(yǎng)基250g/瓶用于植物組織培養(yǎng)incubationmediaRNS培養(yǎng)基250g/瓶用于植物組織培養(yǎng)
黃素 3.0mg/支*5 每支添加于225ml HBJ005中制成LB1肉湯
SoybeanCaseinDigestMedium
金黃色葡萄球菌顯色培養(yǎng)基 1000mL/瓶 incubation media 金黃色葡萄球菌顯色培養(yǎng)基 1000mL/瓶
肉浸液肉湯 250g 用于金黃色葡萄球菌、溶血性鏈球菌和蠟樣芽孢桿菌的培養(yǎng)(GB標(biāo)準(zhǔn))
瓊脂培養(yǎng)基HAgarmediumH(MacConkeyagar)用于腸道致病菌的選擇性分離培養(yǎng)。
LBSagar
胞內(nèi)活化的Notch1蛋白抗體費(fèi)用 伊紅美藍(lán)瓊脂(EMB) 1000(g) incubation media 伊紅美藍(lán)瓊脂(EMB) 1000(g)
營(yíng)養(yǎng)肉湯(NB) 250(g) incubation media 營(yíng)養(yǎng)肉湯(NB) 250(g)
營(yíng)養(yǎng)肉湯(NB) 1000(g) incubation media 營(yíng)養(yǎng)肉湯(NB) 1000(g)
營(yíng)養(yǎng)瓊脂(NA) 250(g) incubation media 營(yíng)養(yǎng)瓊脂(NA) 250(g)
|
|
|
|
|
