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| 產(chǎn)地 | 進(jìn)口、國產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號 | CS9760 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 |
| CAS編號 | |
| 抗體名 | Anti-NOR1 |
| 克隆性 | |
| 靶點(diǎn) | 詳見說明書 |
| 適應(yīng)物種 | 詳見說明書 |
| 形態(tài) | 詳見說明書 |
| 宿主 | 詳見說明書 |
| 亞型 | IgG |
| 標(biāo)識物 | 詳見說明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated synthetic peptide derived from human NOR1 |
產(chǎn)品訂購信息：
英文名稱 Anti-NOR1
中文名稱 神經(jīng)元衍生孤兒受體1抗體說明書
別 名 CHN; Chondrosarcoma, extraskeletal myxoid, fused to EWS; CSMF; MINOR; Mitogen induced nuclear orphan receptor; Mitogen-induced nuclear orphan receptor; Neuron derived orphan receptor 1; Neuron derived orphan receptor; Neuron-derived orphan receptor 1; NOR1; Nr4a3; NR4A3_HUMAN; Nuclear hormone receptor NOR-1; Nuclear hormone receptor NOR1; Nuclear receptor subfamily 4 group A member 3; TEC; Translocated in extraskeletal chondrosarcoma.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Rabbit
產(chǎn)品類型 一抗
研究領(lǐng)域 細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 表觀遺傳學(xué)
蛋白分子量 predicted molecular weight: 68kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human NOR1
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
神經(jīng)元衍生孤兒受體1抗體說明書 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Nur77 (also designated NGFI-B), Nurr1 (Nur-related factor 1), and NOR-1 (neuron-derived orphan receptor 1) constitute the NGFI-B subfamily within the nuclear receptor superfamily. Ligands for these protein have not been identified, and, therefore, they are designated “orphan nuclear receptors”. Genes of the NGFI-B subfamily are classified as immediate-early genes, which are induced rapidly, but transiently, in response to a variety of stimuli. They have been implicated in cell proliferation, differentiation, and apoptosis. The human NOR-1 gene maps to chromosome 9q, and encodes a protein which is expressed in heart, skeletal muscle, thymus, and spleen as well as in brain, where it is developmentally regulated. There-fore, NOR-1 may be involved in regulating neural differentiation. The NOR-1 gene also undergoes chromosomal translocation with the EWS gene to produce a protein thought to affect pre-mRNA splicing.
Function : Binds to the B1A response-element.Subcellular Location : Nucleus.Tissue Specificity : Isoform alpha is highly expressed in skeletal muscle. Isoform beta is highly expressed in skeletal muscle and low expressed in fetal brain and placenta.DISEASE : Defects in NR4A3 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving NR4A3 is found in patients with Erwing sarcoma. Translocation t(9;22)(q22-31;q11-12) with EWSR1. Note=A chromosomal aberration involving NR4A3 is a cause of a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with TAF2N.Similarity : Belongs to the nuclear hormone receptor family. NR4 subfamily.Contains 1 nuclear receptor DNA-binding domain.Database links : UniProtKB/Swiss-Prot: Q92570.3
Anti-VIP receptor II/FITC 熒光素標(biāo)記腺苷酸環(huán)化酶激活肽受體-II/活性腸肽受體-II抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rabbit Anti-mouse IgM/Biotin 生物素化兔抗小鼠IgMMulti-class antibodies規(guī)格： 0.3ml
趨化因子配體1抗體 anti-cx3cl1 0.1ml
Rabbit anti-MG IgG/PE-Cy7 PE-Cy7標(biāo)記的兔抗長爪沙鼠IgG 0.1ml
FABP5 英文名稱： 脂肪酸結(jié)合蛋白5抗體(上皮細(xì)胞型) 0.1ml
Rhesus antibody Rh phospho-VEGFR2 (Tyr1175) 磷酸化內(nèi)皮生長因子受體2抗體 規(guī)格 0.1ml
Rabbit Anti-mouse IgM/Biotin 生物素化兔抗小鼠IgMMulti-class antibodies規(guī)格： 0.3ml
IL-6R Alpha (IL6R-alpha) 白細(xì)胞介素6受體α抗原Multi-class antibodies規(guī)格： 0.5mg
Anti-Adducin protein 內(nèi)收蛋白抗體Multi-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh Phospho-cdc25C (Ser216) 磷酸化細(xì)胞分裂周期蛋白25C抗體 規(guī)格 0.1ml
非水溶性封 10ml 使用后組織收縮較明顯
WBP2 英文名稱： WBP2蛋白抗體 0.2ml
DDX23 英文名稱： 三磷酸腺苷依賴解旋酶DDX23抗體 0.2ml
Anti-Adducin protein 內(nèi)收蛋白抗體Multi-class antibodies規(guī)格： 0.2ml
SNAP23 英文名稱： 突觸相關(guān)蛋白23抗體 0.2ml
Exportin 5 英文名稱： 核輸出蛋白5抗體 0.2ml
巨噬細(xì)胞炎性蛋白3α抗體 Anti-CCL20/MIP3αMacrophage inflammatory ptotein 3 alpha 0.1ml
Anti-VAMP-1/FITC 熒光素標(biāo)記囊泡相關(guān)膜蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh Phospho-HER4 (Tyr984) 磷酸化HER4抗體 規(guī)格 0.1ml
CD14(cluster of differentiation antigen 14) CD14/內(nèi)毒素受體抗原Multi-class antibodies規(guī)格： 0.5mg
BrilliantGreenAgarw/Sulfadi
ISPMedium
改良BPLS瓊脂 BPLS Agar，Modified 250 用于各種標(biāo)本中沙門氏菌選擇性分離培養(yǎng)(ISO標(biāo)準(zhǔn))
Dey/Engley中和肉湯基礎(chǔ)250g/瓶用于化妝品樣品的制備和衛(wèi)生環(huán)境中細(xì)菌的培養(yǎng)，每升培養(yǎng)基中需添加0204incubationmediaDey/Engley中和肉湯基礎(chǔ)250g/瓶用于化妝品樣品的制備和衛(wèi)生環(huán)境中細(xì)菌的培養(yǎng)，每升培養(yǎng)基中需添加0204
HektoenEntericAgar
MCMedium
精氨酸葡萄糖斜面瓊脂(AGS) 用于霍亂弧菌的復(fù)合生化試驗(yàn)(SN標(biāo)準(zhǔn))
胰化蛋白胨 Tryptone 250克 BR
TGY肉湯培養(yǎng)基250g/瓶用于芽孢菌的培養(yǎng)incubationmediaTGY肉湯培養(yǎng)基250g/瓶用于芽孢菌的培養(yǎng)
SOCMedium
神經(jīng)元衍生孤兒受體1抗體說明書 350ML培養(yǎng)袋 規(guī)格： 15x30cm（10個(gè)/袋） 用途： 可重復(fù)使用；使用350ml厭氧產(chǎn)氣劑（C-11）一袋；可裝2-4個(gè)培養(yǎng)皿。
完全釋放型采樣拭子 規(guī)格： 50支/盒 用途： 用于采集、保存和轉(zhuǎn)送食品、環(huán)境及臨床標(biāo)本至微生物實(shí)驗(yàn)室
HK-Swab采集運(yùn)送裝置(Cary-Blair) 規(guī)格： 50支/盒 用途： 改良Cary-Blair運(yùn)送培養(yǎng)基
HK-Swab采集運(yùn)送裝置(改良Stuart) 規(guī)格： 50支/盒 用途： 改良Stuart運(yùn)送培養(yǎng)基
抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：
1. 神經(jīng)元衍生孤兒受體1抗體說明書 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；
2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；
3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；
4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；
5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；
6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及*基,也可與糖基共價(jià)結(jié)合；
7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；
8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。
抗體的鑒定：
1）神經(jīng)元衍生孤兒受體1抗體說明書 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。
2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。
如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。
3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。
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