|
| 產(chǎn)地 | 進(jìn)口、國(guó)產(chǎn) |
| 品牌 | 上海莼試 |
| 保存條件 | Store at -20 °C |
| 貨號(hào) | CS9626 |
| 應(yīng)用范圍 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
| CAS編號(hào) | |
| 抗體名 | Anti-Phospho-NFKB2(Ser866+Ser870) |
| 克隆性 | |
| 靶點(diǎn) | 詳見說(shuō)明書 |
| 適應(yīng)物種 | 詳見說(shuō)明書 |
| 形態(tài) | 詳見說(shuō)明書 |
| 宿主 | 詳見說(shuō)明書 |
| 亞型 | IgG |
| 標(biāo)識(shí)物 | 詳見說(shuō)明書 |
| 濃度 | 1mg/1ml% |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human NF-κB2 p100 around the phosphorylation site of Ser866/870 |
中文名稱 磷酸化細(xì)胞核因子/k基因結(jié)合核因子p100抗體規(guī)格
英文名稱 Anti-Phospho-NFKB2(Ser866+Ser870)
別 名 NFKB2(phospho S869/S870); NFKB2(phospho Ser869/Ser870); p-NFKB2(S869/S870); p-NFKB2(Ser869/Ser870); DNA binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10; Lyt10; Oncogene Lyt 10; DNA binding factor KBF2; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10; Lymphocyte translocation chromosome 10 protein; Lyt 10; Lyt10; NFKB2; NFKB2_HUMAN; Nuclear factor NF kappa B p100 subunit; Nuclear factor NF kappa B p52 subunit; Nuclear factor NF-kappa-B p52 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt 10; Oncogene Lyt-10; p49/p100.
產(chǎn)品屬性：
磷酸化細(xì)胞核因子/k基因結(jié)合核因子p100抗體規(guī)格 濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg
抗體來(lái)源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat
產(chǎn)品類型 一抗 磷酸化抗體
研究領(lǐng)域 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 50/100kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human NF-κB2 p100 around the phosphorylation site of Ser866/870
亞 型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
磷酸化細(xì)胞核因子/k基因結(jié)合核因子p100抗體規(guī)格 產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.
Function : NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.Subunit : Component of the NF-kappa-B RelB-p52 complex. Homodimer; component of the NF-kappa-B p52-p52 complex. Component of the NF-kappa-B p65-p52 complex. Component of the NF-kappa-B p52-c-Rel complex. NFKB2/p52 interacts with NFKBIE. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Directly interacts with MEN1.Subcellular Location : Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).Post-translational modifications : While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing.Constitutive processing is tightly suppressed by its C-terminal processing inhibitory domain, named PID, which contains the death domain.DISEASE : Note=A chromosomal aberration involving NFKB2 is found in a case of B-cell non Hodgkin lymphoma (B-NHL). Translocation t(10;14)(q24;q32) with IGHA1. The resulting oncogene is also called Lyt-10C alpha variant.Note=A chromosomal aberration involving NFKB2 is found in a cutaneous T-cell leukemia (C-TCL) cell line. This rearrangement produces the p80HT gene which encodes for a truncated 80 kDa protein (p80HT).Note=In B-cell leukemia (B-CLL) cell line, LB40 and EB308, can be found after heterogeneous chromosomal aberrations, such as internal deletions.Similarity : Contains 7 ANK repeats.Contains 1 death domain.Contains 1 RHD (Rel-like) domain.Database links : UniProtKB/Swiss-Prot: Q00653.4
實(shí)驗(yàn)流程：
全、新、優(yōu)、品、好四大特點(diǎn)：
磷酸化細(xì)胞核因子/k基因結(jié)合核因子p100抗體規(guī)格 全：公司提供上萬(wàn)種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。
新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。
優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。
好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒有任何的后顧之憂。
技術(shù)外包服務(wù):
★磷酸化細(xì)胞核因子/k基因結(jié)合核因子p100抗體規(guī)格 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等
★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等
★病毒包裝：腺病毒、慢病毒等
★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。
正在熱銷的相關(guān)產(chǎn)品：
ERK(Extracellular signal-regulated kinase) 原活化蛋白激酶抗原Multi-class antibodies規(guī)格： 0.5mg
精氨酸加壓素受體2抗體 Anti-AVPR2 0.1ml
Rhesus antibody Rh Phospho-EGFR (Tyr1101) 磷酸化表皮生長(zhǎng)因子受體抗體 規(guī)格 0.1ml
1640培養(yǎng)液 250ml 國(guó)產(chǎn)
ZNT8 英文名稱： 鋅轉(zhuǎn)運(yùn)體8蛋白抗體 0.2ml
DTNB 英文名稱： 肌Dystrobrevin β蛋白抗體 0.2ml
精氨酸加壓素受體2抗體 Anti-AVPR2 0.1ml
ER- Alpha(phospho-Tyr537)peptide 磷酸化雌激素受體α抗原Multi-class antibodies規(guī)格： 0.5mg
極光激酶B抗體 Anti-Aurora B 0.1ml
Rhesus antibody Rh phospho-EGFR (Thr693) 磷酸化表皮生長(zhǎng)因子受體抗體 規(guī)格 0.1ml
D-MEM/F-12培養(yǎng)基(原裝) 10×1L Gibco
ZNF532 英文名稱： 鋅指蛋白532抗體 0.2ml
DR3 英文名稱： 死亡受體3抗體 0.1ml
極光激酶B抗體 Anti-Aurora B 0.1ml
ZSWIM3 英文名稱： ZSWIM3蛋白抗體 0.2ml
DERP6 英文名稱： 表皮源性蛋白6抗體 0.2ml
載脂蛋白H抗體 Anti-ApoH 0.1ml
Anti-AFP/FITC 熒光標(biāo)記鼠抗人甲胎蛋白單克隆抗體IgGMulti-class antibodies規(guī)格： 0.2ml
Rhesus antibody Rh phospho-EPH A3+A4+A5 ( Tyr779) 磷酸化內(nèi)皮細(xì)胞受體蛋白酪氨酸激酶A3+A4+A5抗體 規(guī)格 0.1ml
DP-I/DSP(desmoplakin I ) 橋粒斑蛋白1抗原Multi-class antibodies規(guī)格： 0.5mg
Sodiumlaurylsulfateagar
Dichloran Rose-Bengal Chloramphenicol Agar Dichloran 1.00466.0500 MERCK默克 incubation media Dichloran Rose-Bengal Chloramphenicol Agar Dichloran 1.00466.0500 MERCK默克
GVC增菌液 250g 用于椰毒假單胞菌酵米面亞種的增菌培養(yǎng)基
C57BL/6小鼠脂肪間質(zhì)干細(xì)胞成脂誘導(dǎo)分化培養(yǎng)基C57BL/6mouseadiposemesenchymalstemcellsintotheculturemediuminduceddifferentiationoffat
TrypticaseSoy-YeastExtractBroth
LM-250g用于濾膜法單增李氏菌選擇性分離(NEOGEN標(biāo)準(zhǔn))
馬尿酸培養(yǎng)基 Ma niu sodium medium 用于彎曲桿菌的馬尿酸水解試驗(yàn)(GB標(biāo)準(zhǔn))
SS瓊脂 Salmonella Shigella Agar 250克 沙門氏菌屬和志賀氏菌屬的分離培養(yǎng)
HTM培養(yǎng)基250g/瓶用于副嗜血桿菌紙片擴(kuò)散法藥敏試驗(yàn)，每升培養(yǎng)基中需添加無(wú)菌NADincubationmediaHTM培養(yǎng)基250g/瓶用于副嗜血桿菌紙片擴(kuò)散法藥敏試驗(yàn)，每升培養(yǎng)基中需添加無(wú)菌NAD
biochemicalidentifi
磷酸化細(xì)胞核因子/k基因結(jié)合核因子p100抗體規(guī)格 庖肉培養(yǎng)基基礎(chǔ) 規(guī)格： 250g 用途： 用于厭氧菌的增菌培養(yǎng)和保存，還用于肉毒梭菌及兼性厭氧和厭氧梭狀芽孢桿菌的檢驗(yàn)(GB/T 8538-200...
動(dòng)力—鹽培養(yǎng)基(A法) 規(guī)格： 250g 用途： 用于鑒別細(xì)菌動(dòng)力和鹽還原試驗(yàn)(GB/T 8538-2008，GB/T4789.28－2003中4.72 ，GB/T4789.14－20...
納氏試劑 規(guī)格： 10ml/瓶 用途： 用于綠膿桿菌分解乙酰胺產(chǎn)試驗(yàn)。
氧化酶試紙 規(guī)格： 10片/盒 用途： 用于氧化酶試驗(yàn)。
|
|
|
|
|
